Composite

Part:BBa_K3396010:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)


GFPnano-FKBP-P2A-Trim21-FRB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


Design Notes

We set this part to realize expressing BBa_K3396002 and BBa_K3396003 into the same cell through transforming only one kind of plasmid.

Source

The original Trim21 is expressed in human. We did the truncation based on the protein domain information given in Uniprot.FRB is from human mTOR and FKBP is from the survey on literature. The sequence of GFP-nano we use came from a patent.P2A is used to express two peptides with only one RBS.

References